Cryptic diversity of Synchaeta spp. (Rotifera, Monogononta) in mountain lakes: relationships with environmental parameters

Cryptic species (i.e. distinct species that are morphologically similar) may have different ecological requirements and their merging into one species can bias the assessment of the main ecological drivers of biodiversity. We investigated the environmental parameters influencing the occurrence of Synchaeta species (S. pectinata, S. grandis, S. lakowitziana, S. tremula/oblonga - monogonont rotifers) in 17 waterbodies of the Trentino-South Tyrol region in the Eastern Alps. To improve taxonomic resolution in phylogenetic analysis, a marine Synchaeta (i.e. S. cf. cecilia) from the United Kingdom was also sampled and sequences from GenBank were downloaded. While Synchaeta species were morphologically identified based on trophi structure, cryptic species were identified based on the generalised mixed Yule coalescent (GMYC) model. We performed multivariate ordination both for morphospecies (i.e. unresolved complexes of cryptic species), a common practice in limnological studies based on morphological taxonomy, and for putative cryptic species, made possible by DNA taxonomy. We expected that resolving complexes of cryptic species could provide more information than using morphospecies. Out of the overall phylogenetic tree with 48 haplotypes from 203 individuals, the GMYC model indicated the presence of 14 GMYC entities, 11 from lakes in the Eastern Alps, 2 from Canada, and one from the U.K. Synchaeta pectinata showed five GMYC entities and S. tremula showed two; all individuals, for which the identification to S. tremula or S. oblonga was not possible, clustered in one monophyletic clade, here called S. tremula/oblonga, that showed additional evidence of three GMYC entities. Environmental-based multivariate ordination on cryptic species explained a significantly higher proportion of variance than that based on morphospecies. Occurrence of putative cryptic species was mainly related to total phosphorus. Moreover, different cryptic species within the same morphospecies showed different, and even opposite, preferences for total phosphorus. In addition, the same GMYC entity of S. tremula/oblonga found in Trentino-South Tyrol was also present in Canada and the same haplotype of S. pectinata found in Trentino-South Tyrol was also found in the U.K. This wide geographical distribution of haplotypes and cryptic species indicated the absence of dispersal barriers in Synchaeta