The formation of enzyme complexes, metabolons, and the channeling of intermediates of secondary metabolism has been discussed for at least 40 years (1). Metabolons and channeling enable plants to perform a highly effective synthesis of specific natural products without or with reduced metabolic interference and to avoid the accumulation of toxic intermediates (2). Despite a long tradition of the concept, precise examples of complete metabolons are scarce. For the anthocyanin pathway, soluble enzymes of the pathway have been shown to interact in yeast cells (3), and to associate with membranes in plant cells (4), the latter presumably through interactions with the membrane anchored P450 hydroxylases of the pathway. The ornamental plant Gerbera hybrida contains flavones, flavonols and anthocyanins in its petals, the exact composition reflecting the color of each gerbera cultivar. Orange, pink and red cultivars contain 4’ hydroxylated pelargonidin derivatives, while magenta and purplish cultivars are rich in 3’, 4’ hydroxylated cyanidin derivatives. Remarkably, 3’, 4’ hydroxylated flavones and flavonols (luteolin and quercetin) can be found in cultivars which completely lack cyanidin. The gerbera cultivar Terra Regina, with orange pelargonidin containing petals, starts to synthesize cyanidin when overexpressing the transcriptional regulator encoding GMYB10 gene, but not in cells where pelargonidin accumulates. These observations are indicative of metabolon control of flavonoid biosynthesis in gerbera petals. We have developed deep transcriptome data using Sanger, 454 and Illumina sequencing of different cultivars of gerbera (5 and unpublished). Assembly of the reads indicates that gerbera expresses small gene families for some of the flavonoid biosynthesis genes (e.g., PAL, 4CL, CHS and CHI) and single genes for others (e.g., DFR and F3’H). We are interested if the corresponding isoenzymes have different roles in biosynthesis of the array of flavonoids present in gerbera, particularly if they are able to form metabolons of different composition and biosynthetic capabilities. In order to map their interaction properties, we are running an all-against-all assay in yeast cells using vectors designed for membrane proteins.
|Citation:||Teeri, T.H.; Bashandy, H.; Martens, S. (2013). Flavonoid metabolons in Gerbera hybrida. In: 7th International Workshop on Anthocyanins, Porto, Portugal, 9-11 September 2012. handle: http://hdl.handle.net/10449/22079|
|Organization unit:||Food Quality and Nutrition Department # CRI_2011-JAN2016|
|Authors:||Teeri, T.H.; Bashandy, H.; Martens, S.|
|Title:||Flavonoid metabolons in Gerbera hybrida|
|Scientific Disciplinary Area:||Settore CHIM/10 - Chimica Degli Alimenti|
|Appears in Collections:||03 - Conference object|