The major phenolic compounds found in apple (Malus x domestica) belong to the class of dihydrochalcones, represented by various phloretin derivatives (e.g. phloridzin, sieboldin, trilobatin). Phloridzin (phloretin 2’-O-glucoside) and the other derivatives are found not only in the apple fruits (peel and seeds) but also in flowers, leaves, roots and bark of the entire tree. Furthermore, significant variations between genotypes and between orchards have been described. Due to the supposed health benefits of these metabolites, knowledge on biochemical and molecular level of the involved proteins/genes is of major importance to support ongoing molecular breeding programmes. The Malus germplasm collection at FEM-IASMA includes around 300 accessions which will be screened for variation in dihydrochalcone profile and content in leaves. From a preliminary screen of the metabolic profile of around 70 accessions it became apparent that three classes can be distinguished: 1. Phloridzin group, 2. Trilobatin, and 3. Sieboldin group. Beside some intermediates all accessions of one group are clearly characterised by the major compound belonging to one or the other group. This knowledge will not only the basis for more detailed characterisation of the biosynthetic pathway but also for isolation of different dihydrochalcones and to prepare specific extracts rich in various combinations of dihydrochalcones for in vitro studies of their function and their bioavailability.
|Citation:||Herrera Valderrama, L.; Malnoy, M.; Ibdah, M.; Barth, S.W.; Martens, S. (2012). Metabolite profiling of dihydrochalcone compounds in apple germplasm collection. In: XXVIth International conference on polyphenols: Polyphenols communications 2012: Florenxe, Italy, 23rd-26th July 2012: 347-348. ISBN: 978-88-907511-0-3. handle: http://hdl.handle.net/10449/21345|
|Organization unit:||Food Quality and Nutrition Department # CRI_2011-JAN2016|
Genomics and Biology of Fruit Crops Department # CRI_2011-JAN2016
|Authors:||Herrera Valderrama, L.; Malnoy, M.; Ibdah, M.; Barth, S.W.; Martens, S.|
|Title:||Metabolite profiling of dihydrochalcone compounds in apple germplasm collection|
Malus x domestica
|Appears in Collections:||03 - Conference object|