Natural resistance to apple proliferation (AP) disease was found in apomictic Malus sieboldii-derived genotypes which can be used as rootstocks for apple. Whereas their agronomic value is currently improved in ongoing breeding programs they are recalcitrant to propagation by standard procedures. Therefore, a micropropagation protocol was developed for in vitro establishment, multiplication and rooting of eleven interesting AP-resistant genotypes. Four different macro and micro element formulations were tested: MS, QL, WPM and DKW. Phytohormones (0.25 µM IBA, 4.44 µM BAP and 0.28 µM GA3) and vitamins (MS modified for thiamine at 2.96 µM), established for the propagation of M. × domestica, were also suitable for the propagation of M. sieboldii genotypes. The MS medium yielded in general the highest proliferation rates and the best shoot growth. A significant improvement of the growth was obtained by replacing Fe-EDTA by Fe-EDDHA as iron source. By comparing four different rooting treatments a significantly higher percentage of rooting was observed when the induction was carried out in the dark with 25 µM IBA either in liquid or agar-solidified medium. Three classes of genotypes with low, medium and high rooting efficiency were found. The acclimatisation method used yielded survival rates between 90-100% for most of the genotypes

Ciccotti, A.M.; Bisognin, C.; Battocletti, I.; Salvadori, A.; Herdemertens, M.; Wallbraun, M.; Jarausch, W. (2009). Micropropagation of Malus sieboldii hybrids resistant to apple proliferation disease. In: Hanke, M.V.; Dunemann, F.; Flachowsky, H. ( editors ) I International symposium on biotechnology of fruit species: BIOTECHFRUIT2008, Dresden, September 1-5, 2008. Leuven: ISHS: 35-42. ISBN: 9789066056121. doi: 10.17660/ActaHortic.2009.839.1 handle: http://hdl.handle.net/10449/19125

Micropropagation of Malus sieboldii hybrids resistant to apple proliferation disease

Ciccotti, Anna Maria;Bisognin, Claudia;Battocletti, Ivana;Salvadori, Antonella;
2009-01-01

Abstract

Natural resistance to apple proliferation (AP) disease was found in apomictic Malus sieboldii-derived genotypes which can be used as rootstocks for apple. Whereas their agronomic value is currently improved in ongoing breeding programs they are recalcitrant to propagation by standard procedures. Therefore, a micropropagation protocol was developed for in vitro establishment, multiplication and rooting of eleven interesting AP-resistant genotypes. Four different macro and micro element formulations were tested: MS, QL, WPM and DKW. Phytohormones (0.25 µM IBA, 4.44 µM BAP and 0.28 µM GA3) and vitamins (MS modified for thiamine at 2.96 µM), established for the propagation of M. × domestica, were also suitable for the propagation of M. sieboldii genotypes. The MS medium yielded in general the highest proliferation rates and the best shoot growth. A significant improvement of the growth was obtained by replacing Fe-EDTA by Fe-EDDHA as iron source. By comparing four different rooting treatments a significantly higher percentage of rooting was observed when the induction was carried out in the dark with 25 µM IBA either in liquid or agar-solidified medium. Three classes of genotypes with low, medium and high rooting efficiency were found. The acclimatisation method used yielded survival rates between 90-100% for most of the genotypes
9789066056121
2009
Ciccotti, A.M.; Bisognin, C.; Battocletti, I.; Salvadori, A.; Herdemertens, M.; Wallbraun, M.; Jarausch, W. (2009). Micropropagation of Malus sieboldii hybrids resistant to apple proliferation disease. In: Hanke, M.V.; Dunemann, F.; Flachowsky, H. ( editors ) I International symposium on biotechnology of fruit species: BIOTECHFRUIT2008, Dresden, September 1-5, 2008. Leuven: ISHS: 35-42. ISBN: 9789066056121. doi: 10.17660/ActaHortic.2009.839.1 handle: http://hdl.handle.net/10449/19125
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