The objective of this protocol is to provide a reliable and replicable method for the sampling of lake micro-phytobenthos and associated microbes in biofilms, to be used in both downstream DNA analysis and algal microscopic counts. The field protocol is optimized for routine sampling and is in agreement with CEN guidance (NF EN 13946) and CEN technical report (CEN/TR 17245) for the analysis of benthic diatoms from rivers and lakes. The application proposed here in the context of EcoAlpsWater aims at comparing DNA inventories to traditional inventories (microscopy).
Rimet, F.; Kurmayer, R.; Salmaso, N.; Capelli, C.; Chardon, C.; Vautier, M.; Gueguen, J.; Bouchez, A.; Domaizon, I. (2021). Updated version- Lake biofilms sampling for both downstream DNA analysis and microscopic counts. url: https://protocols.io/view/updated-version-lake-biofilms-sampling-for-both-do-br2xm8fn.pdf. handle: http://hdl.handle.net/10449/72060
Updated version- Lake biofilms sampling for both downstream DNA analysis and microscopic counts
Salmaso, N.;
2021-01-01
Abstract
The objective of this protocol is to provide a reliable and replicable method for the sampling of lake micro-phytobenthos and associated microbes in biofilms, to be used in both downstream DNA analysis and algal microscopic counts. The field protocol is optimized for routine sampling and is in agreement with CEN guidance (NF EN 13946) and CEN technical report (CEN/TR 17245) for the analysis of benthic diatoms from rivers and lakes. The application proposed here in the context of EcoAlpsWater aims at comparing DNA inventories to traditional inventories (microscopy).
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Rimet_etal_2021_updated version - Lake biofilms.pdf
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