In this chapter details of sampling and sample storage for DNA and RNA analysis are described. Since the sampling strategy and storage protocol are of decisive importance for subsequent analysis, downstream analysis using molecular tools should be defined before the sampling step. This chapter includes protocols to sample planktonic (SOP 2.1) and benthic (SOP 2.2) cyanobacteria as well as a protocol (SOP 2.3) for the isolation of individual filaments/colonies of cyanobacteria under the microscope. The representative sampling of food supplements (SOP 2.4) is described as well. To collect cells for RNA analysis a fast-harvest protocol using low vacuum filtration is described (SOP 2.5). Finally, the SOP 2.6 describes the collecting and recording of sample metadata.

Kurmayer, R.; Christiansen, G.; Kormas, K.; Vyverman, W.; Verleyen, E.; Ramos, V.; Vasconcelos, V.; Salmaso, N. (2017). Sampling and metadata. In: Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria (editor(s) Kurmayer, R.; Sivonen, K.; Wilmotte, A.; Salmaso, N.). Hoboken NJ: Wiley: 19-42. ISBN: 9781119332107 doi: 10.1002/9781119332169.ch2. handle: http://hdl.handle.net/10449/44441

Sampling and metadata

Salmaso, N.
2017-01-01

Abstract

In this chapter details of sampling and sample storage for DNA and RNA analysis are described. Since the sampling strategy and storage protocol are of decisive importance for subsequent analysis, downstream analysis using molecular tools should be defined before the sampling step. This chapter includes protocols to sample planktonic (SOP 2.1) and benthic (SOP 2.2) cyanobacteria as well as a protocol (SOP 2.3) for the isolation of individual filaments/colonies of cyanobacteria under the microscope. The representative sampling of food supplements (SOP 2.4) is described as well. To collect cells for RNA analysis a fast-harvest protocol using low vacuum filtration is described (SOP 2.5). Finally, the SOP 2.6 describes the collecting and recording of sample metadata.
Sample contamination
Sampling strategy
Food supplement
Low-vacuum filtration
Microscopy
Single filament/colony isolation
mRNA analysis
Settore BIO/07 - ECOLOGIA
2017
9781119332107
Kurmayer, R.; Christiansen, G.; Kormas, K.; Vyverman, W.; Verleyen, E.; Ramos, V.; Vasconcelos, V.; Salmaso, N. (2017). Sampling and metadata. In: Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria (editor(s) Kurmayer, R.; Sivonen, K.; Wilmotte, A.; Salmaso, N.). Hoboken NJ: Wiley: 19-42. ISBN: 9781119332107 doi: 10.1002/9781119332169.ch2. handle: http://hdl.handle.net/10449/44441
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/44441
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