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Andreas Ballot, Leonardo Cerasino, Vladyslava Hostyeva, Samuel Cirés
Variability in the sxt Gene Clusters of PSP Toxin Producing Aphanizomenon gracile Strains from Norway, Spain, Germany and North America
www.plosone.org
www.plosone.org
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/F13 1 Tf
1.0998 0 Td
[()-8()20()6(\r)1()4()4(\n)]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.9707 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.1395 0 Td
[(\r)1()12()20(\r)1()]TJ
-34.5087 -1.2983 Td
[()6()11()4(\n)]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.0862 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.0659 0 Td
[()20()6(\r)1()14()4()11()5()12()18()12()]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
4.5637 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.0318 0 Td
[()11()20()4()10()18()12()]TJ
/F7 1 Tf
2.9877 0 Td
(and)Tj
/F13 1 Tf
1.7291 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
0.9751 0 Td
[()3()10()4()4(\n)2()]TJ
/F7 1 Tf
2.4775 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
3.549 0 Td
(The)Tj
/F13 1 Tf
1.7801 0 Td
[()]TJ
/F7 1 Tf
1.3153 0 Td
(gene)Tj
2.0806 0 Td
(cluster)Tj
2.8686 0 Td
(of)Tj
/F13 1 Tf
1.0148 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.0999 0 Td
[()-8()20()6(\r)1()4()4(\n)]TJ
/F7 1 Tf
2.7496 0 Td
(strain)Tj
-33.3749 -1.3039 Td
(NH-5)Tj
2.5511 0 Td
(is)Tj
0.8447 0 Td
(more)Tj
2.3414 0 Td
(similar)Tj
2.9877 0 Td
(in)Tj
1.0375 0 Td
(gene)Tj
2.0806 0 Td
(content)Tj
3.2655 0 Td
(and)Tj
1.7291 0 Td
(cluster)Tj
2.8744 0 Td
(organization)Tj
5.278 0 Td
(to)Tj
1.0262 0 Td
(that)Tj
1.7801 0 Td
(of)Tj
/F13 1 Tf
1.0148 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.1395 0 Td
[(\r)1()12()20(\r)1()12()6()11()4(\n)]TJ
/F7 1 Tf
3.498 0 Td
(than)Tj
2.0296 0 Td
(to)Tj
-35.4782 -1.2983 Td
(that)Tj
1.7744 0 Td
(of)Tj
/F13 1 Tf
1.0148 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.0715 0 Td
[()20()6(\r)1()14()4()11()5()12()18()12()]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
4.5581 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
[()11()20()4()10()18()12()]TJ
/F7 1 Tf
3.8041 0 Td
(or)Tj
/F13 1 Tf
1.0998 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
0.9694 0 Td
[()3()10()4()4(\n)2()]TJ
/F7 1 Tf
2.4832 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
3.5433 0 Td
(Common)Tj
4.0875 0 Td
(to)Tj
1.0205 0 Td
(all)Tj
1.1508 0 Td
(PSP)Tj
1.8142 0 Td
(toxin)Tj
2.3017 0 Td
(producers)Tj
4.2519 0 Td
(is)Tj
0.8504 0 Td
(a)Tj
0.652 0 Td
(set)Tj
-36.4476 -1.2982 Td
(of)Tj
1.0147 0 Td
(14)Tj
1.1679 0 Td
(core)Tj
1.9446 0 Td
(genes)Tj
2.4434 0 Td
(\(e.g.)Tj
/F13 1 Tf
1.8992 0 Td
[()]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.194 0 Td
[(")]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.109 0 Td
[()]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.2337 0 Td
[()]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.211 0 Td
[()]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
1.9899 0 Td
[(#)]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.2337 0 Td
[($)]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.2053 0 Td
[(%)]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.4831 0 Td
[(&)]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.2734 0 Td
[(')]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.126 0 Td
[(\()]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
1.8708 0 Td
[(\))]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
1.8482 0 Td
[(*)]TJ
/F7 1 Tf
(\))Tj
-34.2479 -1.3039 Td
(while)Tj
2.3697 0 Td
(the)Tj
1.4683 0 Td
(composition)Tj
5.2441 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(other)Tj
2.3471 0 Td
(genes)Tj
2.4435 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
/F13 1 Tf
1.474 0 Td
[()]TJ
/F7 1 Tf
1.3096 0 Td
(gene)Tj
2.0806 0 Td
(clusters)Tj
3.2371 0 Td
(varies)Tj
2.5285 0 Td
(between)Tj
3.5263 0 Td
(species)Tj
2.999 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
-34.5427 -1.2983 Td
(22)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(].)Tj
3.2031 0 Td
(The)Tj
1.7858 0 Td
(putative)Tj
3.4412 0 Td
(functions)Tj
3.9855 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(core)Tj
1.9502 0 Td
(genes)Tj
2.4378 0 Td
(include)Tj
3.1861 0 Td
(among)Tj
2.9933 0 Td
(others)Tj
2.71 0 Td
(the)Tj
1.4683 0 Td
(role)Tj
1.7688 0 Td
(of)Tj
1.0148 0 Td
(acyl-carrier)Tj
-32.4281 -1.2983 Td
(protein)Tj
3.1521 0 Td
(\(ACP\),)Tj
3.033 0 Td
(dioxygenase)Tj
5.0967 0 Td
(reductase)Tj
4.0081 0 Td
(or)Tj
1.0999 0 Td
(electron)Tj
3.4639 0 Td
(carrier)Tj
2.88 0 Td
(methylation.)Tj
5.2724 0 Td
(They)Tj
2.2337 0 Td
(are)Tj
1.4513 0 Td
(also)Tj
1.7688 0 Td
(involved)Tj
-33.4599 -1.3039 Td
(in)Tj
1.0318 0 Td
(claisen)Tj
2.9253 0 Td
(condensation,)Tj
5.8393 0 Td
(cyclisation,)Tj
4.6772 0 Td
(desaturation,)Tj
5.4424 0 Td
(amidinotransfer,)Tj
6.9165 0 Td
(C1)Tj
1.355 0 Td
(reduction,)Tj
4.3426 0 Td
(C12)Tj
1.8369 0 Td
(hydrox-)Tj
-34.367 -1.2983 Td
(ylation)Tj
2.9763 0 Td
(and)Tj
1.7291 0 Td
(carbamoylation)Tj
6.4743 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(].)Tj
-9.9835 -1.2982 Td
(Tailoring)Tj
3.9401 0 Td
(genes)Tj
2.4434 0 Td
(\(e.g.)Tj
/F13 1 Tf
1.8992 0 Td
[()]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.16 0 Td
[()]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.0636 0 Td
[(+)]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.2337 0 Td
[(,)]TJ
/F7 1 Tf
(\))Tj
2.2847 0 Td
(are)Tj
1.4514 0 Td
(responsible)Tj
4.7508 0 Td
(for)Tj
1.389 0 Td
(e.g.)Tj
1.559 0 Td
(decarbamoylation,)Tj
7.6478 0 Td
(N-sul-)Tj
-35.0189 -1.2983 Td
(fotransfer,)Tj
4.3369 0 Td
(and)Tj
1.7292 0 Td
(N1-hydroxylation.)Tj
7.6138 0 Td
(Other)Tj
2.5908 0 Td
(genes)Tj
2.4435 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
/F13 1 Tf
1.474 0 Td
[()]TJ
/F7 1 Tf
1.3096 0 Td
(gene)Tj
2.0806 0 Td
(cluster)Tj
2.8743 0 Td
(are)Tj
1.4457 0 Td
(auxiliary)Tj
3.6906 0 Td
(genes)Tj
2.4378 0 Td
(\(e.g.)Tj
/F13 1 Tf
-35.0586 -1.3039 Td
[(-)]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.4094 0 Td
[()23(\n)2()]TJ
/F7 1 Tf
(\),)Tj
3.1804 0 Td
(responsible)Tj
4.7509 0 Td
(for)Tj
1.3889 0 Td
(export)Tj
2.8007 0 Td
(or)Tj
1.0998 0 Td
(regulation.)Tj
4.5354 0 Td
(Genes)Tj
2.6929 0 Td
(with)Tj
1.9956 0 Td
(an)Tj
1.2018 0 Td
(unknown)Tj
4.0819 0 Td
(function)Tj
3.6283 0 Td
(are)Tj
1.4514 0 Td
(e.g.)Tj
/F13 1 Tf
-35.2174 -1.2983 Td
[(.)]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.0862 0 Td
[()]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
2.0863 0 Td
[(/)]TJ
/F7 1 Tf
2.0183 0 Td
(and)Tj
/F13 1 Tf
1.7291 0 Td
[()]TJ
/F7 1 Tf
1.9049 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(].)Tj
2.0749 0 Td
(To)Tj
1.338 0 Td
(date,)Tj
2.1316 0 Td
(the)Tj
1.4683 0 Td
(comparison)Tj
4.9776 0 Td
(of)Tj
1.0148 0 Td
(whole)Tj
/F13 1 Tf
2.6079 0 Td
[()]TJ
/F7 1 Tf
1.3153 0 Td
(gene)Tj
2.0806 0 Td
(clusters)Tj
3.2315 0 Td
(has)Tj
1.542 0 Td
(been)Tj
2.1203 0 Td
(con-)Tj
-35.7276 -1.2982 Td
(ducted)Tj
2.948 0 Td
(on)Tj
1.2755 0 Td
(an)Tj
1.2019 0 Td
(interspecific)Tj
5.1024 0 Td
(level)Tj
2.0125 0 Td
(for)Tj
1.3833 0 Td
(only)Tj
1.9786 0 Td
(one)Tj
1.7008 0 Td
(strain)Tj
2.5001 0 Td
(each)Tj
2.0296 0 Td
(of)Tj
/F13 1 Tf
1.0148 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.1395 0 Td
[(\r)1()12()20(\r)1()12()6()11()4(\n)]TJ
/F7 1 Tf
(,)Tj
/F13 1 Tf
3.7247 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.0942 0 Td
[()-8()20()6(\r)1()4()4(\n)]TJ
/F7 1 Tf
2.7496 0 Td
(NH-5,)Tj
/F13 1 Tf
2.7723 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
-34.6278 -1.3039 Td
[()20()6(\r)1()14()4()11()5()12()18()12()]TJ
/F7 1 Tf
4.3369 0 Td
(T3,)Tj
/F13 1 Tf
1.5307 0 Td
[()2()12()10()18()4()12()4()11()7()10()-4()]TJ
5.1761 0 Td
[()11()20()4()10()18()12()]TJ
/F7 1 Tf
2.9877 0 Td
(D9)Tj
1.4286 0 Td
(and)Tj
/F13 1 Tf
1.7348 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
0.9694 0 Td
[()3()10()4()4(\n)2()]TJ
/F7 1 Tf
2.4832 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
3.5433 0 Td
(No)Tj
1.4626 0 Td
(comparisons)Tj
5.3348 0 Td
(have)Tj
2.0636 0 Td
(been)Tj
2.1203 0 Td
(per-)Tj
-35.172 -1.2983 Td
(formed)Tj
3.1577 0 Td
(on)Tj
1.2756 0 Td
(an)Tj
1.2019 0 Td
(intraspecific)Tj
5.108 0 Td
(level.)Tj
-9.547 -1.2983 Td
(The)Tj
1.7858 0 Td
(known)Tj
3.0047 0 Td
(PSP)Tj
1.8141 0 Td
(toxin)Tj
2.3074 0 Td
(producers)Tj
4.2463 0 Td
(are)Tj
1.4513 0 Td
(characterized)Tj
5.5559 0 Td
(by)Tj
1.1849 0 Td
(varying)Tj
3.2144 0 Td
(toxin)Tj
2.3074 0 Td
(profiles.)Tj
3.4243 0 Td
(Some)Tj
/F13 1 Tf
2.4491 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.0941 0 Td
[()-8()20()6(\r)1()4()4(\n)]TJ
/F7 1 Tf
-35.0359 -1.3039 Td
(strains)Tj
2.8573 0 Td
(mainly)Tj
2.9933 0 Td
(produce)Tj
3.515 0 Td
(neosaxitoxin)Tj
5.3177 0 Td
(\(NEO\),)Tj
3.1748 0 Td
(saxitoxin)Tj
3.8324 0 Td
(\(STX\))Tj
2.6419 0 Td
(and)Tj
1.7291 0 Td
(gonyautoxin5)Tj
5.7203 0 Td
(\(GTX5\))Tj
3.3562 0 Td
(while)Tj
-35.138 -1.2983 Td
(other)Tj
2.347 0 Td
(strains)Tj
2.863 0 Td
(produce)Tj
3.5149 0 Td
(only)Tj
1.9786 0 Td
(STX)Tj
1.9559 0 Td
(and)Tj
1.7348 0 Td
(decarbamoylsaxitoxin)Tj
8.9801 0 Td
(\(dcSTX\))Tj
3.5886 0 Td
(or)Tj
1.0999 0 Td
(NEO)Tj
2.2733 0 Td
(and)Tj
1.7292 0 Td
(STX)Tj
1.9615 0 Td
([)Tj
0.83 0.64 0.02 0 k
(3)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.2586 0 Td
(11)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
-35.2854 -1.2982 Td
(13)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.3946 0 Td
(18)Tj
0 g
(].)Tj
1.7348 0 Td
(In)Tj
1.1112 0 Td
(extracts)Tj
3.2938 0 Td
(of)Tj
/F13 1 Tf
1.0148 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.1395 0 Td
[(\r)1()12()20(\r)1()12()6()11()4(\n)]TJ
/F7 1 Tf
3.5036 0 Td
(STX,)Tj
2.1827 0 Td
(GTX2/3,)Tj
3.7077 0 Td
(N-sulfocarbamoyl)Tj
7.4324 0 Td
(toxins)Tj
2.6645 0 Td
(C1/2,)Tj
2.3924 0 Td
(dcSTX)Tj
2.9084 0 Td
(and)Tj
-34.4804 -1.3039 Td
(decarbamoylgonyautoxin2/3)Tj
11.6729 0 Td
(\(dcGTX2/3\))Tj
5.108 0 Td
(have)Tj
2.0637 0 Td
(been)Tj
2.1203 0 Td
(identified)Tj
4.0591 0 Td
(while)Tj
/F13 1 Tf
2.3698 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.0658 0 Td
[()20()6(\r)1()14()4()11()5()12()18()12()]TJ
/F7 1 Tf
4.337 0 Td
(produces)Tj
-32.7966 -1.2983 Td
(NEO,)Tj
2.4944 0 Td
(dcNEO,)Tj
3.4526 0 Td
(dcSTX)Tj
2.9083 0 Td
(&)Tj
0.9241 0 Td
(STX)Tj
1.9616 0 Td
(and)Tj
/F13 1 Tf
1.7291 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.0375 0 Td
[()11()20()4()10()18()12()]TJ
/F7 1 Tf
2.9877 0 Td
(produces)Tj
3.8777 0 Td
(GTX2/3,)Tj
3.7077 0 Td
(STX)Tj
1.9559 0 Td
(&)Tj
0.9241 0 Td
(low)Tj
1.6611 0 Td
(amounts)Tj
3.7134 0 Td
(of)Tj
-33.3352 -1.2983 Td
(dcGTX2/3)Tj
4.4276 0 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7405 0 Td
(26)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.3946 0 Td
(27)Tj
0 g
(].)Tj
1.7348 0 Td
(The)Tj
1.7858 0 Td
(most)Tj
2.2054 0 Td
(unique)Tj
3.0217 0 Td
(PSP)Tj
1.8142 0 Td
(toxin)Tj
2.3073 0 Td
(profile)Tj
2.8403 0 Td
(is)Tj
0.8448 0 Td
(described)Tj
4.0535 0 Td
(from)Tj
/F13 1 Tf
2.2053 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
0.9751 0 Td
[()3()10()4()4(\n)2()]TJ
/F7 1 Tf
(.)Tj
2.7043 0 Td
(which)Tj
-34.0552 -1.2982 Td
(produces)Tj
/F13 1 Tf
3.8777 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
0.9751 0 Td
[()3()10()4()4(\n)2()]TJ
/F7 1 Tf
2.4775 0 Td
(toxins)Tj
2.6645 0 Td
(\(LWT\))Tj
3.0161 0 Td
(16)Tj
1.6894 0 Td
(not)Tj
1.5761 0 Td
(found)Tj
2.6192 0 Td
(in)Tj
1.0318 0 Td
(other)Tj
2.3527 0 Td
(cyanobacteria)Tj
5.743 0 Td
(to)Tj
1.0261 0 Td
(date,)Tj
2.126 0 Td
(as)Tj
1.0148 0 Td
(well)Tj
1.8255 0 Td
(as)Tj
-34.0155 -1.304 Td
(dcGTX2)Tj
3.6226 0 Td
(and)Tj
1.7348 0 Td
(dcGTX3)Tj
3.6227 0 Td
([)Tj
0.83 0.64 0.02 0 k
(28)Tj
0 g
(].)Tj
-7.7839 -1.2982 Td
(In)Tj
1.1111 0 Td
(Europe,)Tj
3.3619 0 Td
(confirmed)Tj
4.3937 0 Td
(PSP)Tj
1.8141 0 Td
(toxin)Tj
2.3074 0 Td
(producing)Tj
/F13 1 Tf
4.3767 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.0998 0 Td
[()-8()20()6(\r)1()4()4(\n)]TJ
/F7 1 Tf
2.7439 0 Td
(strains)Tj
2.863 0 Td
(have)Tj
2.058 0 Td
(been)Tj
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353.3669 211.9181 l
h
f*
242.0787 172.9134 m
251.7165 172.9134 l
h
f*
389.537 146.948 m
399.0047 146.948 l
h
f*
470.2677 81.9213 m
479.7921 81.9213 l
h
f*
0 g
1 j
1 J
0 w
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1 J
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