Fire blight resistance of the wild apple species Malus fusca (accession MAL0045) has been previously reported. This accession, crossed with the domesticated apple cultivar ‘Idared’, allowed for studies on the genetics of the resistance of this crabapple with the resultant F1 population. A major fire blight locus (Mfu10), found on chromosome 10, explained up to 66% of the phenotypic variance amongst the M. fusca × ‘Idared’ progenies. Although fire blight resistance is strain specific for some Malus accessions, leading to the breakdown of resistance in few resistance donors by highly aggressive strains of Erwinia amylovora; no strain able to breakdown the resistance of M. fusca itself or Mfu10 has been found. This makes this wild apple an interesting model for resistance studies with different wild-type and mutant strains of E. amylovora. A candidate gene (FB_Mfu10), underlying the major locus, was recently proposed. FB_Mfu10 was predicted on the sequence of a bacterial artificial chromosome (BAC) clone, spanning the fire blight locus on chromosome 10 and encodes B-lectin and serine/threonine kinase domains. Preliminary functional analyses showed, that the open reading frame (ORF), together with its border sequences upstream of the start codon and downstream of the stop codon (~ 6000 bp), is present only in resistant F1 genotypes with 8bp distinguishing between susceptibility and resistance. Furthermore, with a dense genetic map of M. fusca and studies with a mutant of an aggressive strain of E. amylovora, a minor fire blight locus has been identified

Peil, A.; Emeriewen, F.; Richter, K.; Malnoy, M. (2019). Malus fusca fire blight resistance: identification of a candidate gene on chromosome 10 and a novel minor locus on chromosome 16. In: ISFB 2019: 2nd International Symposium on Fire Blight of Rosaceous Plants, Traverse city, Michigan, 17-21 July 2019: 60. handle: http://hdl.handle.net/10449/57061

Malus fusca fire blight resistance: identification of a candidate gene on chromosome 10 and a novel minor locus on chromosome 16

Malnoy M.
Ultimo
2019-01-01

Abstract

Fire blight resistance of the wild apple species Malus fusca (accession MAL0045) has been previously reported. This accession, crossed with the domesticated apple cultivar ‘Idared’, allowed for studies on the genetics of the resistance of this crabapple with the resultant F1 population. A major fire blight locus (Mfu10), found on chromosome 10, explained up to 66% of the phenotypic variance amongst the M. fusca × ‘Idared’ progenies. Although fire blight resistance is strain specific for some Malus accessions, leading to the breakdown of resistance in few resistance donors by highly aggressive strains of Erwinia amylovora; no strain able to breakdown the resistance of M. fusca itself or Mfu10 has been found. This makes this wild apple an interesting model for resistance studies with different wild-type and mutant strains of E. amylovora. A candidate gene (FB_Mfu10), underlying the major locus, was recently proposed. FB_Mfu10 was predicted on the sequence of a bacterial artificial chromosome (BAC) clone, spanning the fire blight locus on chromosome 10 and encodes B-lectin and serine/threonine kinase domains. Preliminary functional analyses showed, that the open reading frame (ORF), together with its border sequences upstream of the start codon and downstream of the stop codon (~ 6000 bp), is present only in resistant F1 genotypes with 8bp distinguishing between susceptibility and resistance. Furthermore, with a dense genetic map of M. fusca and studies with a mutant of an aggressive strain of E. amylovora, a minor fire blight locus has been identified
Malus
Fire blight
2019
Peil, A.; Emeriewen, F.; Richter, K.; Malnoy, M. (2019). Malus fusca fire blight resistance: identification of a candidate gene on chromosome 10 and a novel minor locus on chromosome 16. In: ISFB 2019: 2nd International Symposium on Fire Blight of Rosaceous Plants, Traverse city, Michigan, 17-21 July 2019: 60. handle: http://hdl.handle.net/10449/57061
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