Downy mildew (DM), caused by the oomycete Plasmopara viticola (Berk. & Curt.) Berl. & de Toni, is one of the most important plagues affecting viticulture, especially in temperate-humid climates. P. viticola reduces fruit quality and yield, either by direct infection of berries or as a result of the reductions in photosynthesis and plant vigor caused by leaf infections. DM control is based on the repeated and massive use of fungicides, leading to problems such as environmental pollution, development of resistance and residual toxicity. The use of grapevine varieties showing durable resistance to DM is an alternative and promising strategy to control the disease. Nevertheless, most of the in vitro tests developed so far for DM resistance assessment are focused on leaf disk bioassays that not always represent a proper evaluation and prediction of the disease impact on grapevine inflorescence/bunch and therefore on final yield and wine quality. In this work, we firstly improved the annotation procedure of foliar resistance/susceptibility under controlled conditions (optimized OIV descriptor 452-1). Secondly, we developed a new in vitro phenotyping method - from infection to symptom evaluation - for DM resistance assessment on grapevine inflorescence, identifying the most responsive phenological stage (developed and proposed OIV descriptor 453-1). Thus, at this stage we screened several genotypes, in parallel with the optimized leaf disk bioassay, to compare the different pathogen responses between leaf and inflorescence collected from plants in untreated field. Finally, we validated our results performing the same DM resistance assessment also on organs detached from fruiting cuttings grown in phytotrone. Our optimized fruiting cutting agronomic technique turned out to be crucial for the anticipation of the study of late traits under controlled conditions.

Buonassisi, D.; Cappellin, L.; Dolzani, C.; Velasco, R.; Peressotti, E.; Vezzulli, S. (2016). Comparison between grapevine leaf and inflorescence upon downy mildew infection, based on a new in vitro phenotyping method. In: X International Symposium on Grapevine Physiology and Biotechnology, Verona, Italy, 13-18 June 2016: 227 (P199). handle: http://hdl.handle.net/10449/35670

Comparison between grapevine leaf and inflorescence upon downy mildew infection, based on a new in vitro phenotyping method

Buonassisi, Daniele;Cappellin, Luca;Dolzani, Chiara;Velasco, Riccardo;Peressotti, Elisa;Vezzulli, Silvia
2016-01-01

Abstract

Downy mildew (DM), caused by the oomycete Plasmopara viticola (Berk. & Curt.) Berl. & de Toni, is one of the most important plagues affecting viticulture, especially in temperate-humid climates. P. viticola reduces fruit quality and yield, either by direct infection of berries or as a result of the reductions in photosynthesis and plant vigor caused by leaf infections. DM control is based on the repeated and massive use of fungicides, leading to problems such as environmental pollution, development of resistance and residual toxicity. The use of grapevine varieties showing durable resistance to DM is an alternative and promising strategy to control the disease. Nevertheless, most of the in vitro tests developed so far for DM resistance assessment are focused on leaf disk bioassays that not always represent a proper evaluation and prediction of the disease impact on grapevine inflorescence/bunch and therefore on final yield and wine quality. In this work, we firstly improved the annotation procedure of foliar resistance/susceptibility under controlled conditions (optimized OIV descriptor 452-1). Secondly, we developed a new in vitro phenotyping method - from infection to symptom evaluation - for DM resistance assessment on grapevine inflorescence, identifying the most responsive phenological stage (developed and proposed OIV descriptor 453-1). Thus, at this stage we screened several genotypes, in parallel with the optimized leaf disk bioassay, to compare the different pathogen responses between leaf and inflorescence collected from plants in untreated field. Finally, we validated our results performing the same DM resistance assessment also on organs detached from fruiting cuttings grown in phytotrone. Our optimized fruiting cutting agronomic technique turned out to be crucial for the anticipation of the study of late traits under controlled conditions.
Vitis spp.
Disease resistance
Plasmopara viticola
2016
Buonassisi, D.; Cappellin, L.; Dolzani, C.; Velasco, R.; Peressotti, E.; Vezzulli, S. (2016). Comparison between grapevine leaf and inflorescence upon downy mildew infection, based on a new in vitro phenotyping method. In: X International Symposium on Grapevine Physiology and Biotechnology, Verona, Italy, 13-18 June 2016: 227 (P199). handle: http://hdl.handle.net/10449/35670
File in questo prodotto:
File Dimensione Formato  
2016 Vezzulli P199.pdf

accesso aperto

Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.26 MB
Formato Adobe PDF
1.26 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/35670
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact