The aim of the present study was to evaluate the antimicrobial activity of two synbiotic combinations, Lactobacillus fermentum with short-chain fructooligosaccharides (FOS-LF) and Bifidobacterium longum with isomaltooligosaccharides (IMO-BL), against enterohaemorrhagic Escherichia coli O157:H7 and enteropathogenic E. coli O86. Antimicrobial activity was determined (1) by co-culturing the synbiotics and pathogens in batch cultures, and (2) with the three-stage continuous culture system (gut model), inoculated with faecal slurry from an elderly donor. In the co-culture experiments, IMO-BL was significantly inhibitory to both E. coli strains, while FOS-LF was slightly inhibitory or not inhibitory. Factors other than acid production appeared to play a role in the inhibition. In the gut models, both synbiotics effectively inhibited E. coli O157 in the first vessel, but not in vessels 2 and 3. E. coli O86 was not significantly inhibited.

Likotrafiti, E.; Tuohy, K.M.; Gibson, G.R.; Rastall, R.A. (2016). Antimicrobial activity of selected synbiotics targeted for the elderly against pathogenic Escherichia coli strains. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, 67 (2): 83-91. doi: 10.3109/09637486.2015.1134444 handle: http://hdl.handle.net/10449/28337

Antimicrobial activity of selected synbiotics targeted for the elderly against pathogenic Escherichia coli strains

Tuohy, Kieran Michael;
2016-01-01

Abstract

The aim of the present study was to evaluate the antimicrobial activity of two synbiotic combinations, Lactobacillus fermentum with short-chain fructooligosaccharides (FOS-LF) and Bifidobacterium longum with isomaltooligosaccharides (IMO-BL), against enterohaemorrhagic Escherichia coli O157:H7 and enteropathogenic E. coli O86. Antimicrobial activity was determined (1) by co-culturing the synbiotics and pathogens in batch cultures, and (2) with the three-stage continuous culture system (gut model), inoculated with faecal slurry from an elderly donor. In the co-culture experiments, IMO-BL was significantly inhibitory to both E. coli strains, while FOS-LF was slightly inhibitory or not inhibitory. Factors other than acid production appeared to play a role in the inhibition. In the gut models, both synbiotics effectively inhibited E. coli O157 in the first vessel, but not in vessels 2 and 3. E. coli O86 was not significantly inhibited.
Bifidobacterium
Lactobacillus
Gut model
Settore AGR/15 - SCIENZE E TECNOLOGIE ALIMENTARI
2016
Likotrafiti, E.; Tuohy, K.M.; Gibson, G.R.; Rastall, R.A. (2016). Antimicrobial activity of selected synbiotics targeted for the elderly against pathogenic Escherichia coli strains. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, 67 (2): 83-91. doi: 10.3109/09637486.2015.1134444 handle: http://hdl.handle.net/10449/28337
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/28337
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