A steroid 5β-reductase gene corresponding to the hypothetical protein of LOC100247199 from leaves of Vitis vinifera (var. ‘Chardonnay’) was cloned and overexpressed in E. coli. The recombinant protein showed 5β-reductase activity when progesterone was used as a substrate. The reaction was stereo-selective, producing only 5β-products such as 5β-pregnane-3,20-dione. Other small substrates (terpenoids and enones) were also accepted as substrates, indicating the highly promiscuous character of the enzyme class. Our results show that the steroid 5β-reductase gene, encoding an orthologous enzyme described as a key enzyme in cardenolide biosynthesis, is also expressed in leaves of the cardenolide-free plant Vitis vinifera. We emphasize the fact that, on some occasions, different reductases (e.g., progesterone 5β-reductase and monoterpenoid reductase) can also use molecules that are similar to the final products as a substrate. Therefore, in planta, the different reductases may contribute to the immense number of diverse small natural products finally leading to the flavor of wine. KEYWORDS: Vitis vinifera, enone 5β-reductase, secondary metabolites, biosynthesis, gene evolution

Ernst, M.; Munkert, J.; Campa, M.; Malnoy, M.A.; Martens, S.; Müller Uri, F. (2015). Steroid 5β-reductase from leaves of Vitis vinifera: molecular cloning, expression and modelling. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 63 (46): 10112-10120. doi: 10.1021/acs.jafc.5b04261 handle: http://hdl.handle.net/10449/25732

Steroid 5β-reductase from leaves of Vitis vinifera: molecular cloning, expression and modelling

Campa, Manuela;Malnoy, Mickael Arnaud;Martens, Stefan;
2015-01-01

Abstract

A steroid 5β-reductase gene corresponding to the hypothetical protein of LOC100247199 from leaves of Vitis vinifera (var. ‘Chardonnay’) was cloned and overexpressed in E. coli. The recombinant protein showed 5β-reductase activity when progesterone was used as a substrate. The reaction was stereo-selective, producing only 5β-products such as 5β-pregnane-3,20-dione. Other small substrates (terpenoids and enones) were also accepted as substrates, indicating the highly promiscuous character of the enzyme class. Our results show that the steroid 5β-reductase gene, encoding an orthologous enzyme described as a key enzyme in cardenolide biosynthesis, is also expressed in leaves of the cardenolide-free plant Vitis vinifera. We emphasize the fact that, on some occasions, different reductases (e.g., progesterone 5β-reductase and monoterpenoid reductase) can also use molecules that are similar to the final products as a substrate. Therefore, in planta, the different reductases may contribute to the immense number of diverse small natural products finally leading to the flavor of wine. KEYWORDS: Vitis vinifera, enone 5β-reductase, secondary metabolites, biosynthesis, gene evolution
Vitis vinifera
Enone 5β-reductase
Secondary metabolites
Biosynthesis
Gene 28 evolution
Settore BIO/10 - BIOCHIMICA
2015
Ernst, M.; Munkert, J.; Campa, M.; Malnoy, M.A.; Martens, S.; Müller Uri, F. (2015). Steroid 5β-reductase from leaves of Vitis vinifera: molecular cloning, expression and modelling. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 63 (46): 10112-10120. doi: 10.1021/acs.jafc.5b04261 handle: http://hdl.handle.net/10449/25732
File in questo prodotto:
File Dimensione Formato  
2015 JAFC Martens et al.pdf

solo utenti autorizzati

Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 4.09 MB
Formato Adobe PDF
4.09 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/25732
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 2
social impact