Science has just started understanding how the environment drives the composition of microbial communities. Endophytes, as host-associated microbes, respond to environmental stimuli in a host-mediated fashion. To study how temperature and climate may affect endophytic microbial communities we studied grapevine-associated microbial populations using a cultivation independent approach. Grafted cuttings were used to analyse how temperature affects microbial endophytic communities in a controlled environment. The composition of microbial endophytes in the field was assessed by surveying potted plants at different altitudes representing diverse climatic conditions. Seasonal fluctuations in the microbial endophytes were also considered by sampling test plants at different times throughout the year. We adopted a DNA-based, cultivation-independent approach to the analysis of microbial populations variability in the conditions considered in this study. The analysis of DNA amplified by PCR involved the use of both Automated Ribosomal Intergenic Spacer Analysis (ARISA) and Roche 454 GS FLX+ technology.
Pancher, M.; Yousaf, S.; Campisano, A. (2012). Temperature and climate affect the endophytes community in grapevine. In: 14th International Symposium on Microbial Ecology: the power of the small, Copenhagen, Denmark, 19-24 August 2012. url: http://www.isme-microbes.org/isme14/report handle: http://hdl.handle.net/10449/22089
Temperature and climate affect the endophytes community in grapevine
Pancher, Michael;Yousaf, Sohail;Campisano, Andrea
2012-01-01
Abstract
Science has just started understanding how the environment drives the composition of microbial communities. Endophytes, as host-associated microbes, respond to environmental stimuli in a host-mediated fashion. To study how temperature and climate may affect endophytic microbial communities we studied grapevine-associated microbial populations using a cultivation independent approach. Grafted cuttings were used to analyse how temperature affects microbial endophytic communities in a controlled environment. The composition of microbial endophytes in the field was assessed by surveying potted plants at different altitudes representing diverse climatic conditions. Seasonal fluctuations in the microbial endophytes were also considered by sampling test plants at different times throughout the year. We adopted a DNA-based, cultivation-independent approach to the analysis of microbial populations variability in the conditions considered in this study. The analysis of DNA amplified by PCR involved the use of both Automated Ribosomal Intergenic Spacer Analysis (ARISA) and Roche 454 GS FLX+ technology.
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