The Palearctic planthopper Hyalesthes obsoletus is the natural vector of the grapevine yellow disease Bois noir. Grapevine is an occasional host plant of this polyphagous planthopper. To deepen our knowledge of the role of plant volatile organic compounds for H. obsoletus host plant searching, we carried out behavioral, morphological and electrophysiological studies. We tested the attraction of H. obsoletus to nettle, field bindweed, hedge bindweed, chaste tree, and grapevine by using a Y-shaped olfactometer. The results showed a significant attraction of male H. obsoletus to chaste tree, and of the females to nettle. Male H. obsoletus were repelled by odor from hedge bindweed. Ultrastructural studies of the antennae showed at least two types of olfactory sensilla at the antennal pedicel: plaque organs and trichoid sensilla. Volatile organic compounds from nettle and chaste tree were collected, and the extracts were analyzed by coupling gas-chromatography to both mass-spectrometry and electroantennography. The volatile organic compounds that elicited electrophysiological responses in male and female antennae were identified. These findings are discussed with respect to behavior of H. obsoletus males and females in the field.

Riolo, P.; Minuz, R.L.; Anfora, G.; Rossi Stacconi, M.V.; Carlin, S.; Isidoro, N.; Romani, R. (2012). Perception of host plant volatiles in Hyalesthes obsoletus: behavior, morphology, and electrophysiology. JOURNAL OF CHEMICAL ECOLOGY, 38 (8): 1017-1030. doi: 10.1007/s10886-012-0154-2 handle: http://hdl.handle.net/10449/21205

Perception of host plant volatiles in Hyalesthes obsoletus: behavior, morphology, and electrophysiology

Anfora, Gianfranco;Rossi Stacconi, M. V.;Carlin, Silvia;
2012-01-01

Abstract

The Palearctic planthopper Hyalesthes obsoletus is the natural vector of the grapevine yellow disease Bois noir. Grapevine is an occasional host plant of this polyphagous planthopper. To deepen our knowledge of the role of plant volatile organic compounds for H. obsoletus host plant searching, we carried out behavioral, morphological and electrophysiological studies. We tested the attraction of H. obsoletus to nettle, field bindweed, hedge bindweed, chaste tree, and grapevine by using a Y-shaped olfactometer. The results showed a significant attraction of male H. obsoletus to chaste tree, and of the females to nettle. Male H. obsoletus were repelled by odor from hedge bindweed. Ultrastructural studies of the antennae showed at least two types of olfactory sensilla at the antennal pedicel: plaque organs and trichoid sensilla. Volatile organic compounds from nettle and chaste tree were collected, and the extracts were analyzed by coupling gas-chromatography to both mass-spectrometry and electroantennography. The volatile organic compounds that elicited electrophysiological responses in male and female antennae were identified. These findings are discussed with respect to behavior of H. obsoletus males and females in the field.
Planthopper
Phytoplasma vector
Volatile organic compounds
Y-tube bioassays
Electrophysiology
Antenna functional anatomy
Hyalesthes obsoletus
Cairomoni
Settore AGR/11 - ENTOMOLOGIA GENERALE E APPLICATA
2012
Riolo, P.; Minuz, R.L.; Anfora, G.; Rossi Stacconi, M.V.; Carlin, S.; Isidoro, N.; Romani, R. (2012). Perception of host plant volatiles in Hyalesthes obsoletus: behavior, morphology, and electrophysiology. JOURNAL OF CHEMICAL ECOLOGY, 38 (8): 1017-1030. doi: 10.1007/s10886-012-0154-2 handle: http://hdl.handle.net/10449/21205
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