In the codling moth Cydia pomonella (Lepidoptera: Tortricidae) plant volatiles attract males and females by upwind flight and synergise the male response to the female-produced sex pheromone, indicating a close relationship between the perception of social and environmental olfactory signals. We have studied the anatomical and functional organisation of the antennal lobe (AL), the primary olfactory centre, of C. pomonella with respect to the integration of sex pheromone and host-plant volatile information. A three-dimensional reconstruction of the glomerular structure of the AL revealed 50±2 and 49±2 glomeruli in males and females, respectively. These glomeruli are functional units involved in the coding of odour quality. The glomerular map of the AL was then integrated with electrophysiological recordings of the response of individual neurons in the AL of males and females to sex pheromone components and behaviourally active plant volatiles. By means of intracellular recordings and stainings, we physiologically characterised ca. 50 neurons in each sex, revealing complex patterns of activation and a wide variation in response dynamics to these test compounds. Stimulation with single chemicals and their two-component blends produced both synergistic and inhibitory interactions in projection neurons innervating ordinary glomeruli and the macroglomerular complex. Our results show that the sex pheromone and plant odours are processed in an across-fibre coding pattern. The lack of a clear segregation between the pheromone and general odour subsystems in the AL of the codling moth suggests a level of interaction that has not been reported from other insects.

Trona, F.; Anfora, G.; Bengtsson, M.J.; Witzgall, P.; Ignell, R. (2010). Coding and interaction of sex pheromone and plant volatile signals in the antennal lobe of the codling moth Cydia pomonella. JOURNAL OF EXPERIMENTAL BIOLOGY, 213: 4291-4303. doi: 10.1242/jeb.047365 handle: http://hdl.handle.net/10449/19767

Coding and interaction of sex pheromone and plant volatile signals in the antennal lobe of the codling moth Cydia pomonella

Trona, Federica;Anfora, Gianfranco;Bengtsson, Martin Jonas;
2010-01-01

Abstract

In the codling moth Cydia pomonella (Lepidoptera: Tortricidae) plant volatiles attract males and females by upwind flight and synergise the male response to the female-produced sex pheromone, indicating a close relationship between the perception of social and environmental olfactory signals. We have studied the anatomical and functional organisation of the antennal lobe (AL), the primary olfactory centre, of C. pomonella with respect to the integration of sex pheromone and host-plant volatile information. A three-dimensional reconstruction of the glomerular structure of the AL revealed 50±2 and 49±2 glomeruli in males and females, respectively. These glomeruli are functional units involved in the coding of odour quality. The glomerular map of the AL was then integrated with electrophysiological recordings of the response of individual neurons in the AL of males and females to sex pheromone components and behaviourally active plant volatiles. By means of intracellular recordings and stainings, we physiologically characterised ca. 50 neurons in each sex, revealing complex patterns of activation and a wide variation in response dynamics to these test compounds. Stimulation with single chemicals and their two-component blends produced both synergistic and inhibitory interactions in projection neurons innervating ordinary glomeruli and the macroglomerular complex. Our results show that the sex pheromone and plant odours are processed in an across-fibre coding pattern. The lack of a clear segregation between the pheromone and general odour subsystems in the AL of the codling moth suggests a level of interaction that has not been reported from other insects.
Lepidoptera
Olfaction
Intracellular recording
Glomeruli
Blend interaction
Synergism
2010
Trona, F.; Anfora, G.; Bengtsson, M.J.; Witzgall, P.; Ignell, R. (2010). Coding and interaction of sex pheromone and plant volatile signals in the antennal lobe of the codling moth Cydia pomonella. JOURNAL OF EXPERIMENTAL BIOLOGY, 213: 4291-4303. doi: 10.1242/jeb.047365 handle: http://hdl.handle.net/10449/19767
File in questo prodotto:
File Dimensione Formato  
2010 JEB Trona et al.pdf

accesso aperto

Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 3.97 MB
Formato Adobe PDF
3.97 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/19767
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 60
  • ???jsp.display-item.citation.isi??? 59
social impact